A pair of SARS-CoV-2 nucleocapsid protein monoclonal antibodies shows high specificity and sensitivity for diagnosis

An-Kai Zhu,Sa-Sa Li,Shu-Pei Yu,Zhao-Yong Zhang,Huan Li,Jing-Feng Li,Shan-Shan Gao,Heng Chen,Jincun Zhao,Run Shi,Mingjian Lu,Chaoyang Li

Virologica Sinica（2022）

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摘要

•Seven monoclonal antibodies (mAbs) against SARS-CoV-2 nucleocapsid protein are produced, which can be applied in ELISA, WB, and IF assays.•A pair of mAbs, 2G11/bio-1C7, can detect SARS-CoV-2 nucleocapsid protein as low as 15 pg/well in the double sandwich ELISA.•The mAb, 2G11, shows 97.4% sensitivity and 100% specificity for diagnosing the human blood samples.

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