Strategy to synthesize long-wavelength emission carbon dots and their multifunctional application for pH variation and arginine sensing and bioimaging

In this work, we designed N and S co-doped carbon dots (N,S-CDs) with long-wavelength emission and their multifunctional application in pH variation, arginine (Arg) sensing, bioimaging in living cells and zebrafish, and fluorescent materials. The N,S-CDs with excitation wavelength-dependent properties were prepared using neutral red (NR) and dl-methionine (DL-Met) as raw materials by one-pot hydrothermal strategy. The N,S-CDs exhibited a unique pH-sensitive luminescence trait within pH range of 3.2–11.0 and have great linear relationship of 4.8–8.0, which indicating their potential application as an imaging reagent in physiological environments. Arg can quench the PL of N,S-CDs due to static quenching. (SQ). The linear range is 2.5–62.5 μM and the LOD is calculated as 0.68 μM. Furthermore, the as-proposed N,S-CDs can be applied as imaging reagents for monitoring of pH and Arg in vivo and vitro owing to outstanding biocompatibility and low cytotoxicity. Interestingly, the N,S-CDs were also used in fluorescent composite films and phosphors owing to exceptional optical properties. All these results indicate that the N,S-CDs have huge potentiality in the areas of fluorescence sensing, bioimaging and fluorescent materials.