Physiological changes and gene responses during Ganoderma lucidum growth with selenium supplementation.

is highly appreciated for its health and nutrition value. In the present study, was cultivated as selenium transformation carrier, and the physiological changes and gene responses by selenium supplementation were revealed through high-throughput RNA-Seq technology. As a result, selenium supplementation increased the stipe length and the cap size, but decreased the cap thickness of . Mineral salt supplementation could greatly promote the formation of triterpene acids and selenium in . The highest yield was gained in the treatment with selenium content of 200 µg/g. Subsequently, the tissues of at budding and mature stages in this treatment group were sampled for transcriptome analysis and compared to those of a control group without selenium supplementation. A total of 16,113 expressed genes were obtained from the transcriptome of , and GO-annotated unigenes were mainly involved in molecular functions and KEGG-annotated ones were highly expressed in ribosomal pathway. Furthermore, genes involved in carbon metabolism pathway were most promoted by selenium at budding stage of , while gene expression was the highest in the pathway of amino acid biosynthesis at mature stage of . Specially, selenium-related genes in , such as GL23172-G, GL29881-G and GL28298-G, played a regulatory role in oxidoreductase, antioxidant activity and tryptophan synthesis. The results provide a theoretical basis for further study of selenium-enriched mushrooms and aid to development of Se-enriched foodstuff and health products made from fungi.