Ultra-performance convergence chromatography tandem mass spectrometry analysis of adrenal and gonadal steroid hormones in southern white rhinoceros (Ceratotherium simum simum) faeces and serum

•40 steroid hormones analysed in Ceratotherium simum simum serum and faeces.•UPC2-MS/MS analysis targets quantification of adrenal and gonadal steroid metabolites.•Application in the monitoring of stress response and reproductive health in wildlife.•11-deoxycortisol is the major faecal glucocorticoid in southern white rhinoceros.•11-ketoandrostenedione (11KA4) is the only C11-oxy androgen detected in faeces.