Differentiation of Human Adipose-Derived Mesenchymal Stromal/Stem Cells into Insulin-Producing Cells with A Single Tet-Off Lentiviral Vector System.

Hiroyuki Moriyama,Mariko Moriyama,Toshiyuki Ozawa,Daisuke Tsuruta,Takao Hayakawa

Cell journal（2022）

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摘要

Using our single tet-off lentiviral vector system, Pdx-1 and MafA/NeuroD1 could be simultaneously expressed in the absence of Dox. Further, this system allowed the differentiation of hASC into insulin-producing cells.

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关键词

Doxycycline,Gene Expression Regulation,Insulin-producing cells,adipose-derived mesenchymal stromal/stem cells,genetic vectors

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