Impact of buffer composition on biochemical, morphological and mechanical parameters: A tare before dielectrophoretic cell separation and isolation

•DEP is an electrokinetic approach for discriminating and separating floating cells.•Buffer composition differently influence the viability of Caco-2 and K562 cells.•NaCl concentration influences both Flow Cytometry outcomes and cell size.•Buffer composition differently influences cell parameters in Caco-2 and K562 cells.•Buffer composition differently influences gene expression in Caco-2 and K562 cells.