Study on Flavonoids and Bioactivity Features of Pericarp of Citrus reticulata "Chachi" at Different Harvest Periods

Dry mature pericarp of Citrus reticulata "Chachi" (PCR), Pericarpium Citri Reticulatae Chachiensis, is a traditional Chinese medicine that displays characteristics of different usage at different harvest times in clinical use. The corresponding changes in the bioactive components in PCR from different harvest times remain unclear. Therefore, in this study, broadly targeted metabolomics technology was used to compare the differences in bioactive components among pericarps of PCR, which are the raw material of PCR at different growth stages. In the results, 210 kinds of flavonoid metabolites were detected. The content of hesperidin in red PCR harvested in December was higher than that in Citri Reticulatae Pericarpium Viride (CRPV) and reddish PCR harvested from July to November. Furthermore, the content of nobiletin, tangeretin, and 3,3 ',4 ',5,6,7,8-heptamethoxyflavone in CRPV from July to September was higher than that in the PCR harvested at other times. In addition, the result of cluster analysis and PCA showed that CRPV harvested from July to September had an obvious grouping pattern with the reddish PCR and the red PCR harvested from October to December. Differential metabolites in six comparison groups (A1 vs. A6, A1 vs. A2, A2 vs. A3, A3 vs. A4, A4 vs. A5, A5 vs. A6) were 67, 48, 14, 51, 42, and 40, respectively. The common differential metabolite of four comparison groups was 3 ',4 ',7-trihydroxyflavone (A1 vs. A2, A2 vs. A3, A3 vs. A4, A4 vs. A5). All the flavonoid differential metabolites screened were enriched in 16 metabolic pathways. Moreover, the results of the evaluation of the total antioxidant capacity indicated that CRPV in August was a suitable raw material for the production of antioxidants. Through molecular docking, the content of potential anti-SARS-CoV-2 components in the PCR in October was higher than that in the PCR in other periods. These results further proved that PCR at different harvest times was endowed with different efficacy and usage due to the difference in the accumulation of bioactive components.