Biologic: H-Bot Kinematics Based Multi-Micro-Extrusion Bioprinter

Abstract 3D bioprinting offers a novel strategy to create large-scale complex tissue models. Nowadays, layer by layer fabrication is used to create patient specific tissue substitutes. However, commercially available bioprinters cannot be widely used especially in small research facilities due to their high cost, and may not be suitable for bioprinting of complex tissue models. Besides, most of the systems are not capable of providing the required working conditions. The aim of this study is to design and assemble of a low-cost H-Bot based bioprinter that allows multi-micro-extrusion to form complex tissue models in a closed cabin and sterile conditions. In this study, a micro-extrusion based bioprinter, BioLogic, with 3 different print heads namely Universal Micro-Extrusion Module (UMM), Multi Micro-Extrusion Module (MMM) and Ergonomic Multi-Extrusion Module (EMM) were developed. The print heads were tested and scaffold models were bioprinted and analyzed. BioLogic was compared in price with the commercially available bioprinters. Scaffold fabrication was successfully performed with BioLogic. The average pore size of the scaffold was determined as 0.37 ± 0.04 mm (n=20). Total cost of BioLogic was considerably less than any other commercially available bioprinters. A new system is developed for bioprinting of complex tissue models. The cost of the system is appropriate for research and features of the device may be upgraded according to the needs. BioLogic is the first H-Bot kinematics based bioprinter and has ability to measure atmospheric conditions in a closed cabin.