Effect of bovine myosin heavy chain 3 on proliferation and differentiation of myoblast.

The myosin heavy chain 3 () gene is an essential gene that affects muscle development. This study aimed to discuss the expression characteristics of the gene and its effect on the proliferation and differentiation of bovine myoblasts. Quantitative real time-PCR results display that the expression level of was higher in muscle tissue, and the expression increased in the early stage of myoblast differentiation. Interfering with the gene in myoblasts resulted in fewer EDU-positive cells and decreased expression of proliferation marker genes. Interference with can also affect the differentiation process of myoblasts. Regarding phenotype, myotube differentiation in the interference group was slowed or even stopped. Interference with the expression of could significantly reduce the expression of myogenic differentiation marker genes. The above results show that is mainly expressed in muscle tissue and is highly expressed in the early stage of differentiation of bovine myoblasts, and interfering with the can promote the proliferation and inhibit the differentiation of bovine myoblasts. This study provides a theoretical basis for revealing the regulatory process of bovine myoblast proliferation and differentiation and bovine molecular breeding.