Live-cell imaging of the chloroplast outer envelope membrane using fluorescent dyes.

Shintaro Ichikawa,Kazuya Ishikawa,Hitoshi Miyakawa,Yutaka Kodama

Plant direct（2022）

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摘要

We established a live-cell imaging method to visualize the chloroplast outer envelope membrane by staining living cells with fluorescent dyes. This method does not require genetic transformation and allows the observation of the chloroplast outer envelope membrane in various plant species.

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关键词

Nile red,chloroplast,chloroplast outer envelope membrane,confocal laser scanning microscopy,fluorescent dye,live‐cell imaging,rhodamine B,staining,sub‐organellar compartment,various plant species

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