2D-DIGE Analysis of Liver Disease in Mice.
Methods in molecular biology (Clifton, N.J.)(2023)
摘要
Hepatocellular carcinoma (HCC) is the major type of primary liver cancer. In this chapter, we describe our routine two-dimensional difference gel electrophoresis (2D-DIGE) workflow for analysis of mouse liver tissue in physiological conditions, as well as of mouse HCC. 2D-DIGE still constitutes a valuable comparative proteomics technique, not only providing information on global protein expression in a sample but also on potential posttranslational protein modifications, occurrence of protein degradation fragments, and the existence of protein isoforms. Thus, 2D-DIGE analysis provides highly complementary data to non-gel-based shotgun mass spectrometry (MS) methods (e.g., liquid chromatography (LC)-MS/MS)-allowing, for example, identification of novel protein biomarkers for HCC or increasing insights into the molecular mechanisms underlying hepatocarcinogenesis.
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关键词
Comparative proteomics,Hepatocellular carcinoma (HCC),Liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS),Liver cancer,Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF-MS/MS),Mouse tissue,Two-dimensional difference gel electrophoresis (2D-DIGE)
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