A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls.

Konstantinos Sousounis,Katharine Courtemanche,Jessica L Whited

Methods in molecular biology (Clifton, N.J.)（2023）

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摘要

Clustered regularly interspaced short palindromic repeats (CRISPR) is a powerful tool that enables editing of the axolotl genome. In this chapter, we will cover how to retrieve gene sequences, confirm annotation, design CRISPR targets, analyze indels, and screen for Crispant axolotls. This is a comprehensive guide on how to use CRISPR on your favorite gene and gain insights into its function.

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关键词

Axolotl screening,Axolotls,CRISPR,CRISPR design,CRISPR-Cas9,Genome targeting

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