Paenibacillus sabuli sp. nov., isolated from the South China Sea.

A Gram-positive, rod-shaped, motile, spore-forming bacterium, designated strain IB182496, was isolated from coastal sand of the South China Sea. The strain grew optimally at pH 7.0-9.0, 20-30 °C, and with NaCl 3.0-5.0 %. The predominant menaquinone was MK-7 and the major cellular fatty acids were anteiso-C, iso-C and C. The polar lipids in the cell wall included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified phospholipids and one unidentified lipid. The comparison of 16S rRNA gene sequences indicated that strain IB182496 was most closely related to '' SMB1 and SA-7-6 with similarities of 95.7 and 95.5 %, respectively. The whole-genome average nucleotide identity values between strain IB182496 and the two reference strains were 70.8 and 70.5%, and the digital DNA-DNA hybridization values were 18.7 and 18.0 %, respectively. Genomic analyses showed that strain IB182496 presented a genome of 6.22 Mbp with chromosomal G+C content of 60.3 %, and a total of 5261 genes were predicted. The combined phylogenetic relatedness, phenotypic and genotypic features supported the conclusion that strain IB182496 should be considered as representing a novel species of the genus , for which we propose the name sp. nov. with the type strain IB182496 (=MCCC 1K04627=JCM 34216).