Radiosynthesis of 20-[18F]Fluoroarachidonic Acid for PET-MR Imaging: Biological Evaluation in ApoE4-TR Mice

•An increase in brain arachidonic acid (AA) uptake is a marker of calcium-dependent phospholipase A2 activation and neuroinflammation.•A novel translational synthesis approach of 20-[18F]fluoroarachidonic acid ([18F]-FAA) for PET imaging is presented.•[18F]-FAA showed bioequivalent signaling properties to AA in cells.•The brain incorporation coefficient (K*) of [18F]-FAA was estimated via multiple methods in ApoE4 targeted replacement mice using the image derived input function.•The application of [18F]-FAA PET imaging to humans has relevance to identify and guide the effectiveness of treatments focused on neuroinflammation in neurodegenerative diseases.