Preparation and characterization of Bomidin-loaded thermosensitive hydrogel for periodontal application

Periodontitis is a chronic infection and tissue-destructive disease which is considered as the major cause of tooth loss. However, satisfactory clinical treatment for the disease has yet to be achieved. Herein, we designed an injectable thermo-sensitive hydrogel consisting of hydroxypropyl methylcellulose (HPMC), hyaluronic acid (HA) and glycerol (Gl), and further loaded with Bomidin (FP), a recombinant antimicrobial peptide (AMP), the physicochemical and biological characteristics were subsequently investigated in vitro. The results indicated that the prepared hydrogel can be injected and changes phase in situ at desirable temperature. Also, a three-dimensional network structure and cell compatibility were observed. Importantly, the FP-embedded hydrogels exhibited strong antibacterial activities against oral pathogens Porphyromonas gingivalis ( P. gingivalis ), Staphylococcus aureus ( S. aureus ), and Escherichia coli ( E. coli ). These findings indicated our hydrogel possesses not only good fluidity, stable thermosensitivity but also preferable biocompatibility and antibacterial properties, thus demonstrate promising potential for application in periodontal treatment. Trial registration PJ2020-113-001, registration date: Jun 2019. Graphical abstract