ANTI-Trypanosoma cruzi PHENYLPROPANOIDS ISOLATED FROM Baccharis ligustrina C. DC. (ASTERACEAE)

In the present work, dried aerial parts of Baccharis ligustrina (Asteraceae) were subjected to microwave assisted extraction (MAE) using aqueous solution of 1-butyl-3-methylimidazolium bromide (BMImBr) and the obtained extract was successively partitioned using hexane and EtOAc. Using reduced amounts of extracts and efficient chromatographic steps, four acyl C6C3 derivatives (n-hexacosyl ferulate, n-hexacosyl, n-octacosyl, and n-triacontyl p-coumarates) were obtained from hexane phase whereas two C6C3 acids (ferulic and p-coumaric) were obtained from EtOAc phase. Isolated phenylpropanoids were evaluated against amastigote forms of parasite Trypanosoma cruzi. As result, it was observed that p-coumaric and ferulic acids were inactives whereas alkyl derivatives displayed EC50 values of 6.5 mu mol L-1 (n-octacosyl p-coumarate), 9.3 mu mol L-1 (n-triacontyl p-coumarate), 15.7 mu mol L-1 (n-hexacosyl p-coumarate), and 32.2 mu mol L-1 (n-hexacosyl ferulate). All tested compounds displayed reduced toxicity against NCTC cells (CC50 > 200 mmol L-1).