Isothermal Titration Calorimetry Analysis of a Cooperative Riboswitch Using an Interdependent-Sites Binding Model.

Chapin E Cavender,Griffin M Schroeder,David H Mathews,Joseph E Wedekind

Methods in molecular biology (Clifton, N.J.)（2023）

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摘要

Isothermal titration calorimetry (ITC) is a powerful biophysical tool to characterize energetic profiles of biomacromolecular interactions without any alteration of the underlying chemical structures. In this protocol, we describe procedures for performing, analyzing, and interpreting ITC data obtained from a cooperative riboswitch-ligand interaction.

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关键词

Cooperativity,Ligand binding,Nonequivalent-interdependent-sites model,Parabolic thermogram,Riboswitch,Stoichiometry,Thermodynamics

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