Neovascularization-directed bionic eye drops for noninvasive renovation of age-related macular degeneration

Traumatic local therapy and exogenous long-distance fundus drug delivery often cause secondary eye damage and immunogenic inflammation. Herein, inspired by natural neovascular targeting ability of endogenous low-density lipoprotein (LDL), a noninvasive bionic nano-eye-drop with enhanced ocular penetrability and lesion recognizability is developed for the PDT treatment of wAMD. This study presents an attractive non-invasive strategy for the PDT of wAMD. • Bionic eye drops for non-invasive treatment of age-related macular degeneration. • Low-density lipoprotein nano-vector for ocular neovascularization targeted delivery. • Penetratin-mediated enhancement of ocular conjunctival/scleral barrier penetration. • Verteporfin as photosensitizer approved by FDA for ocular photodynamic therapy. • 1000 times less of total drug dosage compare with the current benchmark treatment. The current treatment of wet age-related macular degeneration (wAMD) relies on monthly intravitreal or intravenously injection of vascular endothelial growth factor (VEGF) inhibitor or photodynamic (PDT) agents to inhibit choroidal neovascularization. However, traumatic local therapy and exogenous long-distance fundus drug delivery often lead to secondary eye damage, low treatment efficiency, and immunogenic inflammation. Herein, inspired by the natural neovascular targeting ability of endogenous low-density lipoproteins (LDL), a noninvasive bionic nano-eye-drop with enhanced ocular penetrability and lesion recognizability is developed for enabling the PDT treatment of wAMD. Verteporfin (VP) as a laser-induced PDT agent is protected inside the hydrophobic core of reconstituted LDL (rLDL) vectors. 5-carboxyfluorescein (FAM) conjugated ste-penetratin (PEN, a transmembrane peptide) is anchored on the surface of the rLDL carrier, which enabled the nanoparticles (PEN-rLDL-VP) to cross the blood-retina barrier to realizing visual therapy. Following instillation, PEN-rLDL-VP can effectively deliver VP into neovascular that overexpress LDL receptors, which can respond to laser-induced PDT. Only with a single dose of the eye-drop and laser-induced PDT, the VEGF and proinflammatory intercellular adhesion molecule-1 (ICAM-1) proteins are significantly down-regulated in vivo , which implicates the neovascular inhibition and inflammation alleviation. This study presents an attractive non-invasive strategy for the PDT of wAMD.