Indirect Organogenesis from Stem Derived Callus of Dregea Volubilis (L.F) Benth

In vitro propagation methods offer a powerful tool for germplasm maintenance and multiplication. This report established the methodology for callus regeneration of Dregea volubilis from stem explants. The effect of various concentrations and combinations of IAA, NAA, 2,4-D, BAP, KIN and IBA were tested for in vitro callus induction, plant regeneration, and rooting of D. volubilis. The maximum number of greenish, organogenic callus was achieved on the MS medium containing 1.5 mg/L 2,4-D+ 0.5 mg/L KIN and then subsequent shoot regeneration was obtained on MS medium supplemented with 3.0 mg/L KIN from stem derived callus. The highest frequency of shoot regeneration (81.93%) with the maximum number of shoots (10.56 + 0.44) was achieved in this medium. In vitro raised micro shoots showed a 90% response with the maximum number of roots (5.31 ± 0.66) per shoot on MS medium supplemented with 2.5 mg/L IBA after 15 days. Then in vitro rooted plantlets were moved to mud pot bearing soil and vermiculite mixture in 1:1 ratio for hardening and hardened plantlets showed 90% survivability in field conditions. Using this protocol, it is possible to clonally produce viable, uniform and healthy plants with a maximal survival rate that can be used for large scale cultivation, genetic transformation and biopharmaceuticals applications.