Single-cell mass cytometry analysis reveals stem cell heterogeneity

Thulaj Meharwade,Loïck Joumier,Maxime Parisotto,Mohan Malleshaiah

Methods（2022）

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摘要

•Mass cytometry analysis of a panel of 32 key regulatory proteins in embryonic stem cells (ESC) at the single-cell level.•Mass cytometry reveals the distinct cell-to-cell variation of stem cell regulators across ESC culture conditions.•Mass cytometry reveals extensive cross-activation of signaling pathways in common ESC culture conditions.•Mass cytometry distinguishes varying cell state heterogeneity across ESC cultures.

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关键词

Mass cytometry,cytometry by time of flight (CyTOF),Single-cell protein analysis,Cellular heterogeneity,Embryonic stem cells,Pluripotent stem cells

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