Rat Pheochromocytoma PC12 Cells in Culture.
Methods in molecular biology (Clifton, N.J.)（2023）
摘要
PC12 cells serve as a secretory cell model, especially suitable for studying the molecular mechanisms underlying fusion pore kinetics in regulated exocytosis of dense-core vesicles (DCVs). In this chapter, we describe a series of PC12 cell culture procedures optimized for real-time functional assays such as single-vesicle amperometry. In addition, these conditions have been widely used for single-cell biochemical assays such as the proximity ligation assay with immunostaining.
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关键词
Dense-core vesicle,Fusion pore kinetics,Norepinephrine,PC12 cell culture,Proximity ligation assay with immunostaining,Rat pheochromocytoma,Regulated exocytosis,Single-cell biochemistry assay,Single-vesicle amperometry
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