Application of Virtual Fencing for the management of Limousin cows at pasture

•A sustainable use of pasture-based systems requires efficient grazing management.•Virtual Fencing uses acoustic and electric cues to replace physical fences.•Cow's learning ability was tested by setting three different virtual grazing areas.•Results show a significant decrease of sounds and electrical pulses among trials.•Hair cortisol content was not affected by Virtual Fencing management.