Comparative Efficacy of Serological Tests and Molecular Analysis of Bovine Brucellosis in Western Uttar Pradesh

Background: Brucellosis is a major zoonotic disease that affects people all over the world. Metritis, mastitis, repeat breeding, abortion in the last trimester of pregnancy, retention of placenta and reduced milk production are all symptoms of Brucellosis in the female bovines, whereas epididymitis, orchitis and sterility are symptoms in the male animals. In humans flu-like to undulant fever, arthritis, orchitis and epididymitis can be detected. Methods: A total of 470 bovine samples (serum, whole blood, milk samples) were taken from four districts of western Uttar Pradesh. The 262 sera samples were processed and subjected to RBPT, STAT and ELISA for the determination of seroprevalence of brucellosis and 108 whole blood samples were subjected to molecular analysis by PCR for the disease. Result: Out of 262 (203 cattle and 59 buffalo) serum samples, the seroprevalence of brucellosis in 203 cattle serum sample was found to be 11.82%, 16.25% and 8.37%. Whereas, in 59 buffalo serum samples, the seroprevalence was 3.38%, 15.25% and 0.00% by RBPT, STAT and I-ELISA respectively. Out of 108 whole blood samples, 5 were positive in molecular detection by PCR giving a prevalence rate of 1.62%.