Biological Characterization and Quantification of Rambutan ( Nephelium lappaceum L.) Peel Extract as a Potential Source of Valuable Minerals and Ellagitannins for Industrial Applications.

This study extracted ellagitannins from rambutan peel using the Soxhlet technique. The extract was further partitioned and fractionated to get extract rich in ellagitannin and geraniin, respectively. The partitioning of the extract significantly increased total phenolic content (TPC) by 36.3% and its biological properties. Mineral elements such as Ca, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, and Zn were identified in both peel and extract. Ellagitannins such as geraniin and corilagin with metabolites (gallic acid and ellagic acid) were identified as the major compounds. Analysis of antioxidant activities shows that the ellagitannin rich extract is as powerful as vitamin C. Geraniin was the main contributor to the free radical scavenging activity. The study also revealed that extract with a fraction rich in geraniin has antioxidant activity equivalent to commercial geraniin (1.56 ± 0.11 Trolox equivalent g/g). It also showed low cytotoxicity on fibroblast L929 cells, moderate tyrosinase activity, and good efficacy against , , and strains. Successive fractionation of the extract is a promising technique to produce geraniin rich fractions with enhanced antioxidant property. Rambutan peel, as a natural product, is a good source of mineral elements and biologically active compounds for pharmaceutical, nutraceutical, and cosmetic formulations.