Streptococcus humanilactis sp.nov., isolated from healthy nursing mother's breast milk
Archives of Microbiology(2022)
摘要
Two bacterial strains were isolated from the breast milk of two healthy nursing mothers. The isolates were Gram-positive, catalase-negative, coccus-shaped, chain-forming organisms. Analysis of the 16S rRNA gene sequences of strain IMAU99125 T shared 99.7 and 99.6% similarity with Streptococcus mitis ATCC 49456 T and Streptococcus pseudopneumoniae ATCC BAA-960 T , respectively. The nearly complete 16S rRNA gene sequences of IMAU99125 T and IMAU99674 strains were very closely related (with only 0.06% difference between them). Sequence analysis of the gyr B and rpo B genes also indicated that IMAU99125 T was closely related to S. mitis ATCC 49456 T (94.7% and 97.1%, respectively) and S. pseudopneumoniae ATCC BAA-960 T (94.4% and 97.1%, respectively). Average nucleotide identity (ANI) values between strain IMAU99125 T and S. mitis ATCC 49456 T and S. pseudopneumoniae ATCC BAA-960 T , were 93.3% and 92.7%, respectively. Genome-to-genome distance (GGD) values between strain IMAU99125 T and S. mitis ATCC 99125 T and S. pseudopneumoniae ATCC BAA-960 T were 53.4% (50.7–56.0) and 50.4% (47.7–53.0), respectively. The major fatty acids of the strain were C 16:0 (51.4%). On the basis of the results of phenotypic and phylogenetic analyses, we propose that the two strains be classified as representing a novel species of the genus Streptococcus , namely Streptococcus humanilactis sp . nov. The type strain is IMAU99125 T (= GDMCC 1.1876 T = KCTC 21157 T ). The genome of Streptococcus humanilactis sp. nov. is comprised of 2,027,143 bp. The DNA G + C content of the strain is 40.0 mol%.
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关键词
Streptococcus,Breast milk,Polyphasic taxonomy,Novel species
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