Development of an indirect competitive enzyme-linked immunosorbent assay for formononetin and its application in a cell-based assay using MC3T3-E1 cells

Shunsuke Fujii,Tomoe Ohta,Riho Ehama,Mizuki Irikida,Shuichi Nomura,Yukihiro Shoyama,Takuhiro Uto

Food Chemistry（2023）

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摘要

•A new monoclonal antibody against formononetin is prepared and applied to an ELISA.•The ELISA enables accurate analysis of formononetin content in food samples.•The ELISA can be applied for cell-based assays in molecular biology.•The ELISA quantifies the formononetin and ononin levels taken up by MC3T3-E1 cells.•The novel ELISA is a reliable analytical method.

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FMN,icELISA,mAb,ALP,GC–MS,HPLC,UPLC,HSA,KLH,PEG,HRP,E-RDF,MEMα,FBS,PBS,Q-TOF,ESI,DMSO,LOD,CR

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