Characterization of age-related immune features after autologous NK cell infusion: Protocol for an open-label and randomized controlled trial
FRONTIERS IN IMMUNOLOGY(2022)
摘要
BackgroundAging is usually accompanied by functional declines of the immune system, especially in T-cell responses. However, little is known about ways to alleviate this. MethodsHere, 37 middle-aged healthy participants were recruited, among which 32 were intravenously administrated with expanded NK cells and 5 with normal saline. Then, we monitored changes of peripheral senescent and exhausted T cells within 4 weeks after infusion by flow cytometry, as well as serum levels of senescence-associated secretory phenotype (SASP)-related factors. In vitro co-culture assays were performed to study NK-mediated cytotoxic activity against senescent or exhausted T cells. Functional and phenotypic alteration of NK cells before and after expansion was finally characterized. ResultsAfter NK cell infusion, senescent CD28(-), CD57(+), CD28(-)CD57(+), and CD28(-)KLRG1(+) CD4(+) and CD8(+) T-cell populations decreased significantly, so did PD-1(+) and TIM-3(+) T cells. These changes were continuously observed for 4 weeks. Nevertheless, no significant changes were observed in the normal saline group. Moreover, SASP-related factors including IL-6, IL-8, IL-1 alpha, IL-17, MIP-1 alpha, MIP-1 beta, and MMP1 were significantly decreased after NK cell infusion. Further co-culture assays showed that expanded NK cells specifically and dramatically eliminated senescent CD4(+) T cells other than CD28(+)CD4(+) T cells. They also showed improved cytotoxic activity, with different expression patterns of activating and inhibitory receptors including NKG2C, NKG2A, KLRG1, LAG3, CD57, and TIM3. ConclusionOur findings imply that T-cell senescence and exhaustion is a reversible process in healthy individuals, and autologous NK cell administration can be introduced to alleviate the aging.
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关键词
aging,T-cell senescence,T-cell exhaustion,natural killer cells,SASP
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