Extraction Preparation and Anti-Wrinkle Biomarkers from Ilex x wandoensis C. F. Miller and M. Kim Hybrid nov., a New Plant

Ilex x wandoensis and M. Kim hybrid nov. (IW) is a new variety formed through the natural hybridization of Ilex. cornuta Thunb and Iles. integra Thunb. It was first discovered on Wando Island, Korea. IW has not been studied to date; we investigated the industrial application potential of IW leaf for the first time. We prepared hot water and solvent extracts and tested for biological activity. IW extract was confirmed to have antioxidant and anti-wrinkle effects. The hot water extract had a high antioxidant effect, and the hexane, ethyl acetate, and acetone extracts showed excellent elastase inhibitory activity. The HPLC and GC-MS analyses of β-amyrin and erythrodiol identified them as biomarkers for anti-wrinkle activity. Here, we report for the first time the active ingredients in IW leaf, representing a new plant variety, and suggest that IW extract could be developed as a cosmetic material.