Chemical characterization UPLC-ESI-QToF-MSE, antibacterial and antibiofilm potential of Sarcomphalus joazeiro (MART.) Hauenschild

The present study aims to demonstrate the chemical composition of the ethanolic extract of Sarcomphalus joazeiro bark and to evaluate its antibacterial and antibiofilm activity. For that, the ethanolic extract of the stem bark was prepared, and chemical characterization was carried out in UPLC-ESI-QToF-MSE. Minimum inhibitory concen-tration and antibiotic modification test were performed by the microdilution method. Formation and anti -formation assays were evaluated by the crystal violet method. In the chemical characterization, the presence of 24 peaks was observed. Antibacterial activity showed minimum inhibitory concentration >= 2048 mu g/mL. In the evaluations of the potentiating activity, synergism was the most evident. In the formation analysis, it was observed that most strains showed moderate biofilm formation. It was concluded that saponin derivatives are the major secondary metabolites in the analyzed extract and that it presents relevant synergism with the antibiotics studied and a significant ability to inhibit bacterial biofilm formation.