Comparison of the Chemical Characteristics and Toxicity of PM2.5 Collected Using Different Sizes of Cyclones

Cyclone sampling devices have been helpful in assessing the toxic effects of fine particulate matter (PM2.5). The particle collection efficiency of sampling devices is critical. This study investigated the effect of cyclone size on particle size, chemical composition, and particle toxicity. Three cyclones with different inner diameters (12–68 mm) were tested for penetration using an aerodynamic particle sizer, fluorescent polystyrene latex, and a differential mobility analyzer. The elemental and water-soluble ion compositions of the particles collected by different cyclones were compared. An evaluation of the particles’ toxicity was conducted by comparing the results of dithiothreitol (DTT), limulus amebocyte lysate (LAL), and cell exposure assays. The experimental evaluation showed a 50