A targeted metabolomics method for extra- and intracellular metabolite quantification covering the complete monolignol and lignan synthesis pathway

Andrea Steinmann,Katrin Schullehner, Anna Kohl,Christina Dickmeis,Maurice Finger,Georg Hubmann,Guido Jach,Ulrich Commandeur,Marco Girhard,Vlada B Urlacher,Stephan Lütz

Metabolic Engineering Communications（2022）

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摘要

Microbial synthesis of monolignols and lignans from simple substrates is a promising alternative to plant extraction. Bottlenecks and byproduct formation during heterologous production require targeted metabolomics tools for pathway optimization.

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关键词

Lignans,Targeted metabolomics,Metabolite extraction,Metabolic engineering,Heterologous production,Method development

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