Cross-amplified Barcodes on Slides for Spatial Transcriptomics Sequencing
biorxiv(2022)
摘要
Spatial transcriptomics can reveal molecular signatures of tissue at spatial scales, but current technologies cannot integrate excellent accessibility and easy data-decoding. Here, we use oligonucleotides whose number is the square root of the number of spots to generate cross-amplified spatial barcodes on slides using microfluidic technology. This method can obtain microarrays with well-defined barcodes easily and at low cost, without post-decoding, which contributes to the popularization of spatial transcriptomics.
### Competing Interest Statement
The authors have declared no competing interest.
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关键词
spatial transcriptomics,barcodes,slides,cross-amplified
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