Mouse L1s fade with age: a methylation-enforced mechanism for attenuation of L1 retrotransposition potential

Mice harbor ∼2,800 intact copies of the retrotransposon Long Interspersed Element 1 (L1). The in vivo retrotransposition capacity of an L1 copy is defined by both its sequence integrity and epigenetic status, including DNA methylation of the monomeric units constituting young mouse L1 promoters. Locus-specific L1 methylation dynamics during development may therefore elucidate and explain spatiotemporal niches of endogenous retrotransposition, but remain unresolved. Here, we interrogate the retrotransposition efficiency and epigenetic fate of source (donor) L1s, identified as mobile in vivo . We demonstrate that promoter monomer loss consistently attenuates the relative retrotransposition potential of their offspring (daughter) L1 insertions. We also observe that most donor/daughter L1 pairs are efficiently methylated upon differentiation in vivo and in vitro . We employ Oxford Nanopore Technologies (ONT) long-read sequencing to resolve L1 methylation genome-wide and with locus-specific resolution, revealing a distinctive “smile” pattern in methylation levels across the L1 promoter region and thereby elucidating a molecular mechanism potentially underpinning L1 promoter shortening. Together, our results offer a novel perspective on the interplay between epigenetic repression, L1 evolution, and genome stability. ### Competing Interest Statement The authors have declared no competing interest.