alpha-Ketobutyrate Production under Continuous-Flow Conditions Catalyzed by Immobilized L-Methionine gamma-Lyase

L-Methionine gamma-lyase (MGL) is a PLP-dependent enzyme which catalyzes the alpha, gamma-elimination reaction in sulfur containing amino acids, such as L-methionine and L-cysteine. Its major applications are on metabolic restriction of methionine to cancer cells, food industry (cheese flavor) and for the synthesis of D and L-homoalanine. Herein, we present the immobilization and use of this new immobilized biocatalyst under batch and continuous-flow conditions. The best optimized continuousflow condition allowed 89% conversion in 30 min of residence time with good recyclability of the biocatalyst.