In vitro and in vivo efficacy of poly(lactic acid) nanofiber packaging containing essential oils from Ocimum basilicum L. and Ocimum gratissimum L. against Aspergillus carbonarius and Aspergillus niger in table grapes

Apergillus carbonarius and Aspergillus niger are the principal fungi that attack table grapes, and they are responsible for producing and contaminating these fruits with ochratoxin A. Packaging containing essential oils from Ocimum gratissimum L. and Ocimum basilicum L. encapsulated in poly(lactic acid) (PLA) nanofibers were produced, the antifungal and antiocratoxigenic activities against A. carbonarius and A. niger were evaluated in vitro and in vivo, and the effect of these packages on the quality of table grapes was determined. The nanofibers were produced by the Solution Blow Spinning technique and characterized by Scanning Electron Microscopy and Thermogravimetric Analysis. Fungal contamination and ochratoxin A production were significantly controlled by PLA nanofibers containing the essential oils and the physicochemical parameters of the grapes were preserved, preserving the quality and the shelf life of the fruit. Therefore, the active packaging developed herein has po-tential and may be suitable for application in fruits.