Decolonization of carbapenem-resistant Klebsiella pneumoniae from the intestinal microbiota of model mice by phages targeting two surface structures

FRONTIERS IN MICROBIOLOGY(2022)

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摘要
BackgroundKlebsiella pneumoniae is a normal component of the human gastrointestinal tract microbiota. However, in some cases, it can cause disease. Over the past 20 years, the prevalence of antibiotic-resistant bacteria, such as carbapenem-resistant K. pneumoniae (CRKP), has been increasing. Materials and methodsWe attempted to specifically eliminate CRKP from a mouse model with the human intestinal microbiota. To establish humanized microbiota-colonized mice, we administered K64 CRKP-containing human microbiota to germ-free mice by fecal microbiota transplantation. Then, we used two phages, one targeting the capsule (phi K64-1) and one targeting O1 lipopolysaccharide (phi KO1-1) of K64 K. pneumoniae, to eliminate CRKP. ResultsIn untreated control and phi KO1-1-treated K64-colonized mice, no change in CRKP was observed, while in mice treated with phi K64-1, a transient reduction was observed. In half of the mice treated with both phi KO1-1 and phi K64-1, CRKP was undetectable in feces by PCR and culture for 60 days. However, in the other 50% of the mice, K. pneumoniae was transiently reduced but recovered 35 days after treatment. ConclusionCombination treatment with phi K64-1 and phi KO1-1 achieved long-term decolonization in 52.3% of mice carrying CRKP. Importantly, the composition of the intestinal microbiota was not altered after phage treatment. Therefore, this strategy may be useful not only for eradicating drug-resistant bacterial species from the intestinal microbiota but also for the treatment of other dysbiosis-associated diseases.
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关键词
carbapenem-resistant K, pneumoniae (CRKP), phage treatment, microbiota, decolonization, receptor
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