Abstract 035: Novel Metabolites Associated With Blood Pressure During Dietary Protein And Carbohydrate Interventions

Introduction: Dietary protein and carbohydrate intake have been associated with blood pressure (BP). However, molecular mechanisms for these associations are not well studied. Hypothesis: We hypothesize that novel metabolites – intermediate products of metabolic reactions of diet or genetic factors, may play a role in BP regulation during dietary protein and carbohydrate interventions. Methods: In the Protein and Blood Pressure (ProBP) trial, 80 participants were randomly assigned to take 40 grams/day of soy protein, milk protein, or carbohydrate supplementation each for 8 weeks in a cross-over design. We analyzed the associations of serum metabolites with BP at baseline and each dietary intervention, respectively. Age, sex, race, education, smoking, drinking, and body mass index were adjusted in all analyses. ProBP findings were evaluated for replication among 1206 participants of the Bogalusa Heart Study. Results: After Bonferroni correction for 77 independent metabolite clusters, we identified 17 metabolites (1 at baseline, 8 at carbohydrate diet, 4 at milk protein diet, and 5 at soy protein diet) positively associated with BP (P<6.49E-4). Furthermore, 12 of these metabolite-BP associations identified at dietary interventions were replicated in the BHS (P<0.05, Table ). Of the 12 metabolites, glycerol and hexadecadienoate (16:2n6) were associated with BP in previous studies, and all other metabolite-BP associations were novel. Conclusions: Several novel metabolites may play an important role in BP regulation during dietary protein and carbohydrate intake.