Effect of cell culture media on extracellular vesicle secretion from mesenchymal stromal cells and neurons

•MSC culture media with exosome depleted FBS contains a population of vesicles and the population varies between different supplement batches.•Culture media or maturation timepoint did not affect the number of extracellular vesicles secreted by hPSC neurons.•The EV secretion varied greatly between culture rounds even with identical composition of culture media and same cell line.•It is important to include non-conditioned media as negative control in EV experiments.