Evaluation of genetic diversity of sesame by the study of seed storage protein through sds-page

The sesame seed protein was electrophoretically separated through sodium dodecylesulphate polyacrylamide gel electrophoresis (SDS-PAGE). Electrophoretic banding homology among the genotypes was established not only through the banding pattern but also through estimation of dissimilarity percentage in protein profile. The advantage of SDS–PAGE is that the proteins are separated by their molecular weight and it has become one of the most widely used technique for storage protein separation. Freshly harvested seeds of 21 advance lines and their 8 parental lines were taken for the experiment of our study. The genetic diversity and the relationships among the 29 genotypes were evaluated using sodium dodecylesulphate polyacrylamide gel electrophoresis (SDS –PAGE) which shows difference in number of bands, band width and intensity for different genotypes. It was possible to identify as many as 21 different bands and these can be easily visualized. Dendogram of 21 advance lines and their 8 parents based on SDS – PAGE data using dissimilarity percentage was presented here. Mainly two clusters obtained from the dendrogram for all the 29 genotypes where cluster A forms 3 and cluster B forms 6 sub- clusters. Clustering pattern, size and constituents on the basis of electrophoretic banding pattern was independent of the same through the D² analysis on the basis of biochemical parameters and presence of distinct phylogenetic relationship could be recognized among the genotypes. The dissimilarity % in protein profile indicated that the B- 9 vs. adv. line 5, B-9 vs. adv. line 8, R – 9 vs. adv. line 8, adv. Line 14 vs. adv.18, HT-1 vs. adv. 11 would be useful in identifying parents for heterosis breeding.