Oxidative Stress in the Oral Cavity before and After Prosthetic Treatment

BACKGROUND: Metal ions emitted from dental alloys may induce oxidative stress leading to numerous pathological changes. Lipid peroxidation may cause disturbance of structure and function of cell membranes, apoptosis, autophagy, and formation of potentially mutagenic compounds. Products of interaction between reactive oxygen species and biomolecules may be used for evaluation of oxidative stress level. AIM: The aim of this study was to evaluate the influence of the prosthetic dental treatment with metal ceramic restorations on the level of oxidative stress in the oral cavity. MATERIALS AND METHODS: Metal ceramic crowns with copings fabricated by direct metal laser sintering were produced for 35 patients. CoCr dental alloy EOS CobaltChrome SP2 (EOS) was used. Non-stimulated and stimulated saliva samples were collected from the patients before and after the prosthetic treatment. For evaluation of oxidative stress concentration of 8-isoPGF2-alpha was measured by liquid chromatography tandem mass spectrometry. For statistical processing, non-parametric Wilcoxon signed-rank test and Mann–Whitney test were applied. RESULTS: The concentration of isoprostane 8-isoPGF2-alpha in non-stimulated saliva was lower 2 h after fixing the crowns compared to the initial level and statistically significant difference was observed. On the 7th day the concentration of isoprostanes remained significantly lower than the initial one. No significant differences were found in isoprostane concentration in stimulated saliva before and after prosthetic treatment. CONCLUSION: Prosthetic dental treatment leads to decrease in oral oxidative stress.