Role of Substance P in Regulating Micro-Milieu of Inflammation in Alopecia Areata

Background: Alopecia areata (AA) is an autoimmune disease characterized by chronic inflammation, the pathogenesis of which is unknown. Stress is believed to play a role; how-ever, evidence remains insufficient. A recent study showed that substance P (SP) damaged hair follicles by causing neurogenic inflammation, activating perifollicular mast cells, and inducing keratinocyte apoptosis. Objective: We aimed at studying the role of SP in AA pathogenesis. We investigated the SP levels in the lesional scalp tissues and serum. We also studied the effect of SP on the inflam-matory response and hair growth in the outer root sheath (ORS) cells. Methods: We compared the serum levels of SP in 58 AA patients and 28 healthy subjects. Then, we checked the expression of SP and SP receptor, neurokinin-1 receptor (NK-1R) in the scalps of AA patients and healthy controls using immunohistochemical staining. Finally, we analyzed the mRNA expression of inflammatory cytokines and hair growth-related factors in ORS cells. Results: SP and NK-1R expression were markedly higher in the hair follicles and interfol-licular epidermis of the scalp lesions of AA patients. However, there was no statistically significant difference in serum SP levels between controls and patients, regardless of the type of alopecia. SP significantly increased the mRNA expression of inflammatory cyto-kines and decreased hair growth-related growth factors in ORS cells, but the results were not dramatic. Conclusion: SP triggered a localized micro-inflammation in lesional hair follicles, pro-voked an inflammatory response, and inhibited hair growth, thereby confirming the pathogenic role of SP in AA.