Enhanced butanol production in Clostridium acetobutylicum by manipulating metabolic pathway genes

Guangqing Du,Youduo Wu, Wei Kang,Yongping Xu, Shuying Li,Chuang Xue

Process Biochemistry（2022）

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摘要

•A robust strain with enhanced butanol production was metabolically engineered.•The butanol/ABE ratio increased from 63.2 %–87.6 % by knocking out adc gene.•The knockout of adc gene limited butanol production to ∼11 g/L.•Maximum butanol titer of 19.7 g/L was obtained by overexpressing adhE2 gene.

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关键词

Clostridium acetobutylicum,Butanol,Acetoacetate decarboxylase,Acetaldehyde/alcohol dehydrogenase

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