Development of a fluorescent probe hydrolysis-insulated isothermal PCR for rapid and sensitive on-site detection of African swine fever virus

Tianli Zou,Junhua Deng,Xiangdong Li,Shiyin Zhang,Lingyan Chen,Liying Hao, Jinshan Zhuang,Heng Wang,Guihong Zhang,Shengxiang Ge,Kegong Tian

Virologica Sinica（2022）

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摘要

•A probe-based insulated isothermal PCR (iiPCR) assay was developed for rapid and onsite detection of ASFV.•The developed iiPCR showed similar sensitivity and specificity with OIE recommended real-time PCR.•Blood samples could be directly applied as PCR template in iiPCR without DNA extraction.

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