Silver Microelectrode Arrays for Direct Analysis of Hydrogen Peroxide in Low Ionic Strength Samples

Silver microelectrode arrays are fabricated by photolithography for a one-step analysis of H2O2 in low ionic strength samples. The effects of electrode length, width, band-to-band separation, connection height, and adhesion layer are evaluated. The developed sensor shows excellent repeatability (RSD=1.20 % (n=5)) and reproducibility (RSD=1.12 % (n=5)) with the linear range of 0.0-10.0 mM, the sensitivity of 9.84 +/- 0.34 mu A mM(-1), and the detection limit of 22.69 mu M. The sensor has been successfully applied to detect H2O2 directly without the addition of supporting electrolyte in synthetic urine, tap water, drinking water, and milk samples.