Hoxa5 inhibits adipocyte proliferation through transcriptional regulation of Ccne1 and blocking JAK2/STAT3 signaling pathway in mice.

The highly regulated proliferation of adipocytes plays a momentous role in fat development and obesity. is an important member of the Hox family, its encoded protein is an important transcription factor related to development, and its differential expression in different adipose tissues seems to indicate that may be involved in the regulation of adipocyte proliferation. To evaluate the regulatory mechanism of on adipocyte proliferation, we constructed a variety of expression vectors in vivo and in vitro to explore its mechanism on adipocyte proliferation and its potential impact on obesity. We observed that the overexpression of strongly reduces cell counts and can inhibit cell proliferation and block cell cycle progression by regulating the expression of genes such as , and . Most importantly, we demonstrated that exerts its effect by regulating the signaling pathway of Janus kinase 2 (JAK2) signal transduction and transcription 3 (STAT3) activator, as well as binding to the promoter region of Ccne1 and inhibiting the transcription of Ccne1. This study provides an in-depth understanding of the potential molecular mechanism of inhibiting adipocyte proliferation. Our results suggest the importance of in the treatment of obesity.