Vesicle choreographies keep up cell-to-extracellular matrix adhesion dynamics in polarized epithelial and endothelial cells

•Apico-basally polarized cells exploit spatially oriented microtubule tracks to establish and maintain exo-endocytic traffic of integrins and ECM proteins.•Traffic logistics of vesicular cargos relies on sequential agonistic and synchronous antagonistic cooperation between microtubule motors.•Integrin-mediated cell adhesion to the ECM at the basal plasma membrane drives the formation of different protein complexes that capture microtubules +ends and tether exocytic vesicles.