Pseudovibrio flavus sp. nov. isolated from the sea sponge Verongula gigantea .

A Gram-negative, motile, rod-shaped marine bacterium, designated RKSG542, was isolated from the sea sponge collected at a depth of 20 m off the west coast of San Salvador, The Bahamas. Phylogenetic analyses based on 16S rRNA gene and genome sequences place RKSG542 in a monophyletic clade with members of the genus . Strain RKSG542 shared <96.7 % 16S rRNA gene sequence similarity,<72.2 % average nucleotide identity,<66.7 % average amino acid identity, and <24.8 % digital DNA-DNA hybridization with type strains of the family . Growth occurred at 22-37 °C (22-30 °C optimum), at pH 7-9 (pH 7 optimum), and with 0.5-5 % (w/v) NaCl (2 % optimum). The predominant fatty acids (>10 %) were summed feature 8 (C 6 and/or C 7), C and C, and the respiratory lipoquinone was Q-10. The polar lipid composition comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, three unknown aminolipids, six unknown phospholipids and four unknown lipids. The DNA G+C content of the genome sequence was 52.5 mol%. Based on the results of biochemical, phylogenetic and genomic analyses, RKSG542 (=TSD-76=LMG 29867) is presented here as the type strain of a novel species within the genus (family , order , class ), for which the name sp. nov. is proposed.