Electrochemical Biosensors for Soluble Epidermal Growth Factor Receptor Detection

Simple detection of oncomarkers is of paramount importance in cancer diagnosis. Screen-printed carbon electrodes (SPCEs) were modified by streptavidin, which served as a suitable platform for aptamer molecule binding. The electrochemical conditions for soluble epidermal growth factor receptor (EGFR) sensing were studied and optimized. Developed sensors display a wide linear range from 10 to 500 ng/ml and a low limit of detection of 1.19 ng/ml. SPCEs modified by streptavidin could be used not only as an amperometric sensor but also as an impedimetric one. Moreover, SPCEs were successfully tested for soluble EGFR detection in blood serum samples. This platform presents a simple solution for the application of aptamers in electrochemical testing without the multistep working electrode modification processes. Graphical Abstract