A fragment of a 70 kDa Heterodera glycines heat shock protein (HgHSP70) interacts with soybean cyst nematode-resistant protein GmSHMT08
Journal of Integrative Agriculture(2022)
摘要
Soybean cyst nematode (SCN) Heterodera glycines is considered as the major constraint to soybean production. GmSHMT08 at Rhg4 locus on chromosome 08, encoding a serine hydroxylmethyltransferase, is a major gene underlying resistance against H. glycines in Peking-type soybeans. However, the molecular mechanism underpinning this resistance is less well characterized, and whether GmSHMT08 could interact with proteins in H. glycines remains unclear. In this study, yeast two-hybrid screening was conducted using GmSHMT08 as a bait protein, and a fragment of a 70-kDa heat shock protein (HgHSP70) was screened from H. glycines that exhibited interaction with GmSHMT08. This interaction was verified by both GST pull-down and bimolecular fluorescence complementation assays. Our finding reveals HgHSP70 could be applied as a potential candidate gene for further exploring the mechanism on GmSHMT08-mediated resistance against SCN H. glycines.
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