Facile Access to Far-Red Fluorescent Probes with Through-Space Charge-Transfer Effects for In Vivo Two-Photon Microscopy of the Mouse Cerebrovascular System

Much effort has been devoted to the generation of fluorescent probes by synthetic approaches. In this study, we developed a facile strategy to construct far-red fluorescent probes based on through-space charge transfer within complexes of acceptors and donors and their "twist+twist" interactions. Owing to their rare two-photon excitation property, the complexes could be used for in vivo imaging of the mouse cerebrovascular system.